 National University of Ostroh Academy | Ostroh | Public | 1994 | — | — |
 National University of Kiev-Mohyla Academy | Kyiv | Public | 1991 | — | — |
 Lviv University | Lviv | Public | 1661 | — | — |
 Kharkiv National University | Kharkiv | Public | 1805 | — | EngineeringSocial SciencesMedicine |
 Lviv Polytechnic National University | Lviv | Public | 1844 | — | — |
 Taras Shevchenko National University of Kyiv | Kyiv | Public | 1834 | Ukrainian | — |
 Ukrainian National Forestry University | Lviv | Public | 1874 | — | — |
 National Agricultural University | Kyiv | Public | 1898 | — | — |
 National Mining University | Dnipro | Public | 1899 | — | — |
 Odessa State Medical University | Odesa | Public | 1900 | — | — |
 Odessa National Academy of Food Technologies | Odesa | Public | 1902 | — | — |
 Kiev National Economic University | Kyiv | Public | 1906 | — | — |
 Kherson State University | Kherson | Public | 1917 | — | — |
 Odessa National Polytechnic University | Odesa | Public | 1918 | — | — |
 East Ukrainian National University | Dnipro | Public | 1920 | — | — |
 Donetsk National Technical University | Pokrovsk | Public | 1921 | Ukrainian | — |
 Luhansk Taras Shevchenko National Pedagogical University | Poltava | Public | 1921 | — | — |
 National Pirogov Memorial Medical University | Vinnytsia | Public | 1921 | — | — |
 Ukrainian Medical Stomatological Academy | Poltava | Public | 1921 | — | — |
 Dnepropetrovsk National University of Railway Transport | Dnipro | Public | 1930 | — | — |
