 Universidad del Sagrado Corazon | San Juan | Private | 1880 | — |
 Universidad de Puerto Rico | San Juan | Public | 1903 | Spanish, English |
 Universidad Interamericana de Puerto Rico | San Juan | Private | 1912 | — |
 Pontificia Universidad Catolica de Puerto Rico | Ponce | Private | 1948 | — |
 Sistema Universitario Ana G. Méndez | San Juan | Private | 1941 | — |
 Universidad Central de Bayamón | Bayamón | Private | 1961 | — |
 Centro de Estudios Avanzados de Puerto Rico y el Caribe | San Juan | Private | 1968 | — |
 Caribbean University | Bayamón | Private | 1969 | — |
 Universidad del Turabo | Gurabo | Private | 1941 | — |
 Universidad Central del Caribe | Bayamón | Private | 1976 | — |
 San Juan Bautista School of Medicine | Caguas | Private | 1978 | — |
 Universidad de Puerto Rico, Rio Pedras | San Juan | Public | — | — |
 Universidad de Puerto Rico, Mayaguez | Mayagüez | Public | — | — |
 Universidad Interamericana de Puerto Rico, San Germán | San Germán | Private | — | — |
 Universidad Ana G. Méndez | Gurabo | Private | — | — |
 Universidad de Puerto Rico, Humacao | Humacao | Public | — | — |
 Universidad Interamericana de Puerto Rico, Bayamón | Bayamón | Private | — | — |
 Universidad de Puerto Rico, Ciencias Medicas | San Juan | Public | — | — |
 Universidad de Puerto Rico, Arecibo | Arecibo | Public | — | — |
 Universidad de Puerto Rico, Cayey | Cayey | Public | — | — |
